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Nathalie Gorret

ESR Supervisor

N. Gorret first supervisor of ESR 10, academic supervisor of ESR 8 and ESR 9.
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Short CV

Dr. Nathalie Gorret is an INRA Research Scientist in the Fermentation Advances and Microbial Engineering Group at the Toulouse Biotechnology Institute since July 2007. Her pHD (2000) followed by a 3 years post-doctorate at Massachussets Institute of Technology (Cambridge – USA) in the laboratory of Pr Sinskey (Biology Department) have contributed to develop her expertise on microbiology/physiology and biochemical engineering skills. She joined the Toulouse Biotechnology Institute in 2005 to investigate the dynamics of the interaction between biological and physical phenomena observed in large-scale bioreactor. Her researches focus on the understanding of the impact of the heterogeneities (substrates/products/pH/dissolved oxygen gradients) on the microbial behaviour at both population and sub-population level (physiological states / Morphological states / Genetic stability) in order to optimize microbial processes. She develops skills on methodologies and tools to characterize and quantify the transient microbial responses to environmental stresses using offline and online biosensors at both global population and single-cell levels.

Key expertise, mainly used research methodologies, equipment

TBI-FAME provides a complete environment for research in fermentation processes including set of classical bioreactors and specific bioreactors for gas fermentation from 1mL to 10 liters, on-line gas composition analysers, CO2, O2, H2 dissolved probes, analytical equipment for substrates and product identification and quantitation (HPLCs, GCs, GC-MS-MS). The team is also equipped with flow cytometers for single-cell analysis. The team has also access to the different technology platforms at TBI (transcriptomics, proteomics, fluxomics).

Hosting Institution

The “Institut National des Sciences Appliquées” of Toulouse, is an international, pluridisciplinary, state engineering school, recognized for the excellence of its five-year education which attracts students of a high academic level. INSA-Toulouse provides a range of 8 engineering specializations, including biological engineering. Its courses, linked to the latest scientific advances, are supported by the activity of leading-edge research laboratories as the TBI, backed by large industrial groups. The school is part of the seven doctoral schools. It offers 8 Research Master programs and receives around 240 PhD students, half of whom come from abroad.
The Toulouse Biotechnology Institute (TBI) is a mixed research unit (UMR) attached to INRA (UMR 792), CNRS (UMR 5504) and INSA Toulouse. Internationally recognized, the lab was evaluated “Exceptional” in 2014 and graded A+ by AERES in 2009, with three awards under the “Investissements d’Avenir” (Investing in the Future) program, TBI holds an innovative position at the interface between life sciences and process sciences. Its work has applications in the sectors of health, biotechnologies, water and environment, food processing and agri-business, and chemistry.

RELEVANT PUBLICATIONS

  • Barthe M., Tchouanti-fotso J., Gomes P., Bideaux C., Lestrade D., Grahal C., Steyer J. P., Meleard S., Armand J., Gorret N., Cocaign-Bousquet M., Enjalbert B., Metabolic heterogeneity is controlled by the molecular switch XylR during Escherichia coli adaptation from glucose to xylose. Under Press in mBIO.
  • Boy C, Lesage J, Alfenore S, Gorret N, Guillouet SE. 2020. Plasmid expression level heterogeneity monitoring via heterologous eGFP production at the single-cell level in Cupriavidus necator. Appl. Microbiol. Biotechnol. 104:5899-5914.
  • Timoumi A, Bideaux C, Guillouet SE, Allouche Y, Molina-Jouve C, Fillaudeau L, Gorret N. 2017. Influence of oxygen availability on the metabolism and morphology of Yarrowia lipolytica: insights into the impact of glucose levels on dimorphism. Applied Microbiology Biotechnology. doi: 10.1007/s00253-017-8446-7
  • Marc J, Grousseau E, Lombard E, Sinskey AJ, Gorret N, Guillouet SE. 2017. Over expression of GroESL in Cupriavidus necator for heterotrophic and autotrophic isopropanol production. Metabolic engineering 42:74-84.
  • Timoumi A, Cléret M, Bideaux C, Guillouet SE, Allouche Y, Molina-Jouve C, Fillaudeau L, Gorret N. 2016. Dynamic behavior of Yarrowia lipolytica in response to pH perturbations: dependence of the stress response on the culture mode. Applied Microbiology and Biotechnology:1-16.
  • Grunwald S, Mottet A, Grousseau E, Plassmeier JK, Popović MK, Uribelarrea J-L, Gorret N, Guillouet SE, Sinskey A. 2015. Kinetic and stoichiometric characterization of organoautotrophic growth of Ralstonia eutropha on formic acid in fed-batch and continuous cultures. Microbial Biotechnology 8:155-163.
  • Ternon C, Grousseau E, Gunther J, Gorret N, Guillouet S, J. SA, Aceves-Lara CA, Roux G. 2014. Dynamic Model for Isopropanol Production by Cupriavidus Necator. p 4388-4393. 19th IFAC World Congress 2014, Cape Town, South Africa.
  • Grousseau E, Lu JN, Gorret N, Guillouet SE, Sinskey AJ. 2014. Isopropanol production with engineered Cupriavidus necator as bioproduction platform. Applied Microbiology and Biotechnology 98:4277-4290.

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