Chassis strain engineering for efficient gas feedstock conversion

Hydrogen-driven whole-cell biotransformations

Process intensification of gas-driven processes

WP1

Chassis strain engineering for efficient gas feedstock conversion

The objective of WP1 is to provide an optimized strain for the conversion of the gas feedstock by metabolic engineering, flux analysis and the engineering of transport systems. The design of molecular genetic tools and the comprehensive characterization of selected strains will be essential.

ESR1: Synthetic biology to streamline the metabolism of C. necator toward the production of rare amino acids and proteins

1st Supervisor: Dr. Sandy Schmidt
2nd Supervisor: Prof. Lars M. Blank
University of Groningen

ESR2: Metabolic strategies for the synthesis of isotope-labeled amino acids in C. necator

1st Supervisor: Dr. Sandy Schmidt
2nd Supervisor: Dr. Lars Lauterbach
University of Groningen

ESR3: Metabolic Engineering of C. necator to exploid lipid de novo synthesis for OH-fattyacid ester production

1st Supervisor: Prof. Lars M. Blank
2nd Supervisor: Dr. Sandy Schmidt
RWTH Aachen

ESR4: Transport engineering in C. necator

1st Supervisor: Prof. Robert Kourist
2nd Supervisor: Dr. Stéphane Guillouet
acib GmbH

WP2

Hydrogen-driven whole-cell biotransformations

This WP aims to develop an enzymatic cascade reaction that is taking place in a whole-cell conversion with recombinantly expressed enzymes. Genetic tools that have been developed in WP1 will be applied for the expression.
ESR5: H2-driven production of substituted piperidines in C. necator
1st Supervisor: Dr. Lars Lauterbach
2nd Supervisor: Dr. Sandy Schmidt
TU Berlin
ESR6: Engineering of C. necator for H2-driven production of functionalized piperazines
1st Supervisor: Dr. Lars Lauterbach
2nd Supervisor: Prof. Stéphane Guillouet
TU Berlin
ESR7: Electrophotoautotrophic production of N-heterocycles by an enzymatic cascade in Rhodopseudomonas palustris
1st Supervisor: Prof. Ricardo Louro
2nd Supervisor: Dr. Sandy Schmidt
University of Lisbon

WP3

Process intensification of gas-driven processes

In WP3 the training network will develop a high-pressure lab-scale bioreactor for H2/CO2/O2-based reactions under consideration of the safety aspect. The engineered strains of WP1 and WP2 will be tested in this bioreactor and the physiological understanding of autotrophic fermentations will be improved.
ESR8: Impacts of high pressure for efficient biomolecules production from CO2: from reactor design to microbial physiology
1st Supervisor: Prof. Stéphane Guillouet
2nd Supervisor: Prof. Lars M. Blank
INSA Toulouse
ESR9: Impact of gas delivering membrane systems on the production of biomolecules from CO2: from reactor design to microbial physiology
1st Supervisor: Prof. Stéphane Guillouet
2nd Supervisor: Assoc. Prof. Regina Kratzer
INSA Toulouse
ESR10: Dynamic analysis of subpopulation distributions of engineered C. necator for biomolecules production from CO2: determination of the strain robustness under the constraints of gas fermentation
1st Supervisor: Dr. Nathalie Gorret
2nd Supervisor: Prof. Robert Kourist
INSA Toulouse
ESR11: Gas fermentation to match microbial requirements and technological feasibilities
1st Supervisor: Assoc. Prof. Regina Kratzer
2nd Supervisor: Prof. Stéphane Guillouet
acib GmbH